Home
HelpTest Code HEPP Hepatitis Profile
Infectious
Additional Codes
| EPIC | LAB4124 |
Methodology
Roche Cobas e801
Sample Type
| Preferred Sample Type | |
|---|---|
| Yellow top - Serum | |
| Acceptable Sample Type(s) | |
|---|---|
| Red top - Serum | |
| Light green top - Plasma | |
| Dark green top - Plasma | |
Centrifuge: Yes
Specimen Minimum Volume
1 mL
Specimen Stability
| Temperature | Time |
|---|---|
| Ambient (18-25°C) | 3 days |
| Refrigerated (2-8°C) (store) | 14 days |
| Frozen (<-20°C) | 3 months* |
Separate serum or plasma from cells ASAP.
*Samples may be frozen up to 5 times.
Reference Ranges
Hepatitis A IgM Antibody
| HAVAB - Initial Testing | ||
|---|---|---|
| Cut-off Index (COI) | Result | Retest Procedure |
| <0.90 | Non-reactive (negative) | Retesting not required |
| ≥0.90 to <1.10 | Borderline | Retest in duplicate |
| ≥1.10 | Reactive (positive) | Retesting not required |
| HAVAB - Repeat Testing | ||
|---|---|---|
| COI after retest | Final Result | Interpretation |
| 2 of the 3 results are <1.0 | Non-reactive | Negative for HAV IgM antibodies. |
| 2 of the 3 results are ≥1.0 | Reactive | Positive for HAV IgM antibodies. |
| 2 of the 3 results are between ≥0.90 to <1.10 | Borderline | It is recommended that a specimen be drawn in two weeks and retested. |
......................................................................................................................................................................
Hepatitis B Surface Antigen
| HBSAG - Initial Testing | |||
|---|---|---|---|
| Cut-off Index (COI) | Qualitative Result | Interpretation | Retest Procedure |
| <0.90 | Non-reactive (Negative) | HBsAg not detected | Retesting not required |
| ≥ 0.90 to < 1.0 | Borderline | Borderline zone (undetermined) | All initially reactive or borderline samples should be retested in duplicate. |
| ≥ 1.0 | Reactive (Positive) | HBsAg detected | |
| HBSAG - Repeat Testing | |||
|---|---|---|---|
| Initial Result | Result after retest | Final Result | Interpretation |
| Borderline | 2 of the 3 results have a COI <1.0 | Non-reactive | HBsAg not detected |
| 2 of the 3 results have a COI ≥ 1.0 | Reactive | Presumptive evidence of HBV. Test will be sent out for confirmation.* | |
| Reactive | 2 of the 3 results have a COI <1.0 | Non-reactive | HBsAg not detected |
| 2 of the 3 results have a COI ≥ 1.0 | Reactive | Presumptive evidence of HBV. Test will be sent out for confirmation.* | |
*Repeat values that are Reactive (>1) will reflex the mayo sendout HBAG for confirmation.
......................................................................................................................................................................
Hepatitis B Core IgM Antibody
| HBCAB - Initial Testing | |||
|---|---|---|---|
| Cut-off Index (COI) | Result | Interpretation | Retest Procedure |
| <0.9 | Non-reactive (Negative) | No HBc IgM antibodies detected | Retesting not required |
| ≥0.9 to <1.1 | Borderline | Borderline zone (undetermined) | Retest in duplicate. |
| ≥1.1 | Reactive (Positive) | HBc IgM antibodies detected | Retesting not required |
| HBCAB - Repeat Testing | |||
|---|---|---|---|
| Initial Result | Result after retest | Final Result | Interpretation |
| Borderline | 2 of the 3 results are <1.0 | Non-reactive | No HBc IgM antibodies detected |
| 2 of the 3 results are ≥1.0 | Reactive | HBc IgM antibodies detected | |
......................................................................................................................................................................
Hepaitis C IgG Antibody
| HEPC - Initial Testing | |||
|---|---|---|---|
| Cut-off Index (COI) | Result | Interpretation | Retest Procedure |
| <0.90 | Non-reactive (Negative) | HCV antibodies not detected | Retesting not required |
| ≥0.90 to < 1.0 | Borderline | Borderline zone (undetermined) | Retest in duplicate |
| ≥1.0 | Reactive (Positive) | HCV antibodies detected | Retesting not required, presumptive evidence of antibodies to HCV. Test will be sent out for confirmation.* |
| HEPC - Repeat Testing | ||
|---|---|---|
| COI after retest | Final Result | Interpretation |
| 2 of the 3 results have a COI <1.0 | Non-reactive | HCV antibodies not detected |
| 2 of the 3 results have a COI ≥1.0 | Reactive | Presumptive evidence of antibodies to HCV. Test will be sent out for confirmation.* |
*Reactive (positive) results will reflex the mayo sendout HCVQN for confirmation.
Rejection Criteria
| Hemolysis | Index >1,000 |
|---|---|
| Icterus | Index >40 |
| Lipemia | Index >2,000, ultracentrifuge |
Availability
| Performed | STAT |
|---|---|
| 24/7 | Yes |
Performing Laboratory
MultiCare Yakima Memorial Hospital Laboratory
Lab Department
Chemistry
CPT Code
80074
LOINC
24363-4 Acute hepatitis 2000 panel (S)