Test Code ELU Acid Elution of Antibodies (Eluate)
Additional Codes
Reflex only
Clinical Significance
Elution is a procedure for the recovery of antibody bound to red blood cells. Eluates can be used to identify antibodies coating the red cells of patients who have a positive direct antiglobulin test (DAT).
Methodology
Gamma ELU-KIT II
Sample Type
| Preferred Sample Type | |
|---|---|
| Pink top - EDTA Whole blood | |
| Acceptable Sample Type(s) | |
|---|---|
| Lavender top - EDTA Whole blood | |
Centrifuge: Yes (In Blood Bank)
Specimen Minimum Volume
3 mL whole blood (1 mL of packed RBCs)
Specimen Stability
| Temperature | Time |
|---|---|
| Ambient (18-25°C) | 8 hours |
| Refrigerated (2-8°C) (store) | 72 hours |
| Frozen (-15 to -20°C) | Do not freeze |
Rejection Criteria
| Hemolysis | N/A |
|---|---|
| Icterus | N/A |
| Lipemia | N/A |
Clot samples are not suitable for acid elution testing.
Availability
| Performed | STAT |
|---|---|
| 24/7 | No |
Performing Laboratory
MultiCare Yakima Memorial Hospital Laboratory
Lab Department
Blood Bank
CPT Code
86860
LOINC
897-9 Blood group antibody screen Elution QI