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Test Code ELU Acid Elution of Antibodies (Eluate)

Additional Codes

Reflex only

Clinical Significance

Elution is a procedure for the recovery of antibody bound to red blood cells. Eluates can be used to identify antibodies coating the red cells of patients who have a positive direct antiglobulin test (DAT).

Methodology

Gamma ELU-KIT II

Sample Type

Preferred Sample Type
Pink top - EDTA Whole blood

 

Acceptable Sample Type(s)
Lavender top - EDTA Whole blood

Centrifuge: Yes (In Blood Bank)

Specimen Minimum Volume

3 mL whole blood (1 mL of packed RBCs)

Specimen Stability

Temperature Time
Ambient (18-25°C) 8 hours
Refrigerated (2-8°C) (store) 72 hours
Frozen (-15 to -20°C) Do not freeze

 

Rejection Criteria

Hemolysis N/A
Icterus N/A
Lipemia N/A

Clot samples are not suitable for acid elution testing.

Availability

Performed STAT
24/7 No

 

Performing Laboratory

MultiCare Yakima Memorial Hospital Laboratory

Lab Department

Blood Bank

CPT Code

86860

LOINC

897-9 Blood group antibody screen Elution QI

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