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HelpTest Code DAT Direct Antiglobulin Test
Additional Codes
| EPIC | LAB4410 |
Synonyms
- DAT
- Direct Coombs
Clinical Significance
The direct antiglobulin test is used to demonstrate in vivo coating of red cells with globulin, in particular IgG and C3d. Washed red cells from a patient or donor are tested directly with anti-human globulin (AHG) reagents.
Methodology
MTS Gel card system and tube hemagglutination method
Sample Type
| Preferred Sample Type | |
|---|---|
| Pink top - Whole Blood | |
| Acceptable Sample Type(s) | |
|---|---|
| Lavender top - Whole Blood | |
Centrifuge: Yes (In Blood Bank)
Specimen Minimum Volume
1 mL
NOTE: More sample is required if an eluate is to be performed.
Specimen Stability
| Temperature | Time |
|---|---|
| Ambient (18-25°C) | 72 hours |
| Refrigerated (2-8°C) (store) | 72 hours |
| Frozen (-15 to -20°C) | N/A |
Reference Ranges
Normal
Negative
Abnormal
Positive
Rejection Criteria
| Hemolysis | Reject |
|---|---|
| Icterus | N/A |
| Lipemia | N/A |
Availability
| Performed | STAT |
|---|---|
| 24/7 | Yes |
Performing Laboratory
MultiCare Yakima Memorial Hospital Laboratory
Lab Department
Blood Bank
CPT Code
86880
LOINC
13946-9 Complement+Immunoglobulin QI (RBC)