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Test Code CSFDIF Differential, CSF

Infectious

Purpose

Cytocentrifuge is used to concentrate cells within a defined area to enable morphological identification and differential counting. It provides more cells thatn that which would be present in a wedge smear preparation on the same sample and therefore, provides for greater precision in counting. The principle of cytocentrifugation is that the cell concentration is denser than the suspending fluid and under applied force the cell will have greater momentum than the fluid. The cellular movement occurs after passing through the sample chamber. The cells are projected towards the microscopic slide with sufficient momentum to cross the exit port formed by placing a filter card between the chamber sample and the glass slide resulting in cell to slide adhesion.

Methodology

Wright stain with microscopic examination and review

Sample Type

Sterile CSF tubes (Tube #4 is preferred)

Specimen Minimum Volume

1 mL

Specimen Stability

Temperature Time
Ambient (18-25°C) 2 hours
Refrigerated (2-8°C) 24 hours
Frozen (-15 to -20°C) N/A

Reference Ranges

WBCs Normal Range
Adults Neonates
Lymphocytes 40 - 80% 5 - 35%
Monocytes 15 - 45% 50 - 90%
Neutrophils 0 - 6% 0 - 8%

Rejection Criteria

Hemolysis N/A
Icterus N/A
Lipemia N/A

 

Availability

Performed STAT
24/7 Yes

 

Performing Laboratory

MultiCare Yakima Memorial Hospital Laboratory

Lab Department

Hematology

CPT Code

89051

LOINC

29584-0 Differential panel (CSF)