Test Code BFDIF Differential, Body Fluid
Purpose
Cytocentrifuge is used to concentrate cells within a defined area to enable morphological identification and differential counting. It provides more cells thatn that which would be present in a wedge smear preparation on the same sample and therefore, provides for greater precision in counting. The principle of cytocentrifugation is that the cell concentration is denser than the suspending fluid and under applied force the cell will have greater momentum than the fluid. The cellular movement occurs after passing through the sample chamber. The cells are projected towards the microscopic slide with sufficient momentum to cross the exit port formed by placing a filter card between the chamber sample and the glass slide resulting in cell to slide adhesion.
Methodology
Wright stain with microscopic examination and review
Sample Type
- Amniotic Fluid
- Pleural Fluid
- Pericardial Fluid
- Synovial Fluid
Specimen Minimum Volume
1 mL
Specimen Stability
Temperature | Time |
---|---|
Ambient (18-25°C) | 8 hours |
Refrigerated (2-8°C) | 24 hours |
Frozen (-15 to -20°C) | N/A |
Reference Ranges
WBCs | Normal Range |
---|---|
Lymphocytes | 0 - 75% |
Monocytes | 0 - 70% |
Neutrophils | 0 - 25% |
Rejection Criteria
Hemolysis | N/A |
---|---|
Icterus | N/A |
Lipemia | N/A |
Availability
Performed | STAT |
---|---|
24/7 | Yes |
Performing Laboratory
MultiCare Yakima Memorial Hospital Laboratory
Lab Department
Hematology
CPT Code
89051
LOINC
29580-8 Differential panel (Body fld)