Test Code BCTPCR Bacterial PCR, UW

Additional Codes
EPIC | LAB5897 |
UW | BCTDNA |
Purpose
Accurate identification of bacterial isolates is an essential task of the clinical microbiology laboratory that enables initiaion of proper antimicrobial therapy. For many organisms, traditional phenotypic identification may be difficult, laborious and time consuming. This issue is further confounded by phenotypic variation within species, many newly described pathogenic species and the limited battery of phenotypic tests available to distinguish among established and potential bacterial pathogens. It is sometimes essential to identify bacterial isolates to species level in order to rule out species that are drug resistant or more pathogenic, to detect unsuspected pathogens, ascribe pathogenicity to species so far considered to be nonpathogenic and to identify new bacterial species.
Methodology
DNA extraction, nucleic acid purification, polymerase chain reaction, sequencing.
Sample Type
- Tissue
- Fluid (non-blood)
Specimen Minimum Volume
1 mL
Specimen Stability
Freeze specimens in sterile containers at -20°C.
Rejection Criteria
- Fluids collected in citrated or heparinized solutions.
- Direct blood, serum, plasma, and stool specimens.
- Tissues floating in excess formalin.
Performing Laboratory
University of Washington Laboratory
CPT Code
87801
LOINC
76575-0 Bacterial 16s rRNA NAA+probe QI (Specimen)