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Test Code VAGPAN Vaginitis Panel

Infectious

Additional Codes

EPIC LAB4567

Purpose

The Affirm VPIII Microbial Identification Test is a DNA probe test intended for use in the detection and identification of Candida species, Gardnerella vaginalis and Trichomonas vaginalis nucleic acid in vaginal fluid specimens from patients with symptoms of vaginitis/vaginosis.

Methodology

Nucleic acid hybridization (BD Affirm VPIII)

Sample Type

Affirm VPIII Sample Collection Kit

Includes

  • BD Affirm VPIII Sterile Swab
  • Transport tube with cap.
  • 0.3 mL Ambient Temperature Transport System (ATTS) reagent dropper.

 

Collection Instructions

Vaginal Sample Collection

  1. Label the Sample Collection Tube (SCT) with the patient identification information and collection time.
  2. Place patient in position for a pelvic examination. Insert a speculum into the vagina to permit visualization of the posterior vaginal fornix.
  3. Using the sterile swab, obtain a sample from the posterior vaginal fornix. Twist or roll the swab against the vaginal wall two or three times, ensuring the entire circumference of the swab has touched the vaginal wall. Swab the lateral vaginal wall while removing the swab.
  4. Immediately place the swab into the Sample Collection Tube (SCT).
  5. With the swab touching the bottom of the collection tube, grasp the pre-scored handle of the swab just above the top of the tube and bend until the swab breaks.
  6. Place the cap over the exposed end of the swab and firmly press the cap onto the tube. The cap will "snap" onto the tube when it is properly seated.

Specimen Stability

With ATTS Reagent

Temperature Time
Ambient (15-30°C) 72 hours
Refrigerated (2-8°C) (store) 72 hours
Frozen (-15 to -20°C) N/A

 

Without ATTS Reagent*

Temperature Time
Ambient (15-30°C) 1 hour
Refrigerated (2-8°C) 4 hours
Frozen (-15 to -20°C) N/A

*Analyze without delay.

Result Reporting

Positive Result - Nucleic acid detection for Candida species, Gardnerella, and/or Trichomonas.

 

Negative Result - No nucleic acid detection for Candida species, Gardnerella, or Trichomonas.

Rejection Criteria

  • Specimen collection with materials outside of Affirm VPIII collection kit.
  • Samples outside of defined stability limits.

Availability

Performed STAT
24/7 Yes

 

Performing Laboratory

MultiCare Yakima Memorial Hospital Laboratory

Lab Department

Microbiology

CPT Code

87480

87510

87660

LOINC

92699-8 Candida sp DNA Probe QI (Vag fld)

92698-0 G. vaginalis DNA Probe QI (Vag fld)

92700-4 T. vaginalis DNA Probe QI (Vag fld)