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Test Code LIPA Lipase

Additional Codes

EPIC LAB4136

Clinical Significance

Lipase measurements are used primarily in the diagnosis and treatment of pancreatic disorders.

 

Source: Beckman Coulter Unicel, LIP IFU. REF #476851 12/22/15

Methodology

The random access lipase reages utilizes the methodology of Panteghini to determine pancreatic lipase activity in serum and plasma. The SYNCHRON system(s) monitors the rate of formation of methylresorufin which forms spontaneously from two couples reactions which utilize a 1,2-O-diauryl-rac-glycero-3-glutaric acid-(6'-methylresorufin)-ester as a substrate. The measured rate of color formation at 560 nm is directly proportional to the pancreatic lipase activity.

Sample Type

Preferred Sample Type
Light green top - Plasma

 

Acceptable Sample Type(s)
Dark green top - Plasma
Yellow top - Serum
Red top - Serum

Centrifuge: Yes

Specimen Minimum Volume

0.3 mL

Specimen Stability

Temperature Time
Ambient (18-25°C) 4 hours
Refrigerated (2-8°C) (store) 48 hours
Frozen (-15 to -20°C) 1 month

Separate plasma or serum from cells within 2 hours.

Reference Ranges

Normal Range
22 - 51 U/L

 

Technical Range
10 - 400 U/L

Rejection Criteria

Hemolysis N/A
Icterus N/A
Lipemia 4+ or greater, ultracentrifuge

 

Availability

Performed STAT
24/7 Yes

 

Performing Laboratory

MultiCare Yakima Memorial Hospital Laboratory

Lab Department

Chemistry

CPT Code

83690

LOINC

3040-3 Lipase [Catalytic activity/Vol]

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