Methodology

The cLac electrode is an amperometric electrode consisting of a silver cathode with AgCl reference band and a platinum anode, all protected by an electrode jacket filled with electrolyte solution. At the tip of the jacket is a multilayer membrane. In the lactate electrode, an electrical chain is established between the platinum wire and the Ag/AgCl reference band via the electrolyte. Lactate molecules are transported across the outer membrane to be converted by the enzyme to form H₂O₂. The H₂O₂ is then oxidized to oxygen and electrons, a current measured by an ammeter. The amount of current produced is proportional to the amount of H₂O₂, which is turn is directly related to the amount of lactic acid in the sample.