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HelpTest Code DLDL LDL Cholesterol, Direct
Additional Codes
| EPIC | LAB5225 |
Clinical Significance
LDL cholesterol is directly related to the risk of developing coronary heart disease. A low HDL/LDL cholesterol ratio is directly related to the risk of developing coronary artery disease. Elevated LDL cholesterol is the primary target of cholesterol-lowering therapy.
Source: Beckman Couler Unicel, LDLD IFU. REF #969706 12/22/15
Methodology
This direct LDL cholesterol method is a homogeneous assay without the need for any off-line pretreatment or centrifugation steps. The method depends on a unique detergent which solubulizes only the non-LDL lipoprotein particles and releases cholesterol to react with cholesterol esterase and cholesterol oxidase to produce a non-color forming reaction. A second detergent solubilizes the remaining LDL particles, and a chromogenic coupler allows for color formation. (Beckman Coulter UniCel DxC 660i)
Sample Type
| Preferred Sample Type | |
|---|---|
| Light green top - Plasma | |
| Acceptable Sample Type(s) | |
|---|---|
| Dark green top - Plasma | |
| Yellow top - Serum | |
| Red top - Serum | |
Centrifuge: Yes
Specimen Minimum Volume
0.3 mL
Specimen Stability
| Temperature | Time |
|---|---|
| Ambient (18-25°C) | 8 hours |
| Refrigerated (2-8°C) (store) | 48 hours |
| Frozen (-15 to -20°C) | 1 month |
Separate plasma or serum from cells within 2 hours.
Reference Ranges
| Normal Range |
|---|
| <100 mg/dL |
| Technical Range |
|---|
| 10 - 550 mg/dL |
Sample results are not reported if the triglyceride level is >1293 mg/dL.
Rejection Criteria
| Hemolysis | N/A |
|---|---|
| Icterus | N/A |
| Lipemia | N/A |
Availability
| Performed | STAT |
|---|---|
| 24/7 | Yes |
Performing Laboratory
MultiCare Yakima Memorial Hospital Laboratory
Lab Department
Chemistry
CPT Code
83721
LOINC
18262-6 Cholesterol in LDL Direct assay [Mass/Vol]